Chichester City bowed out of Sussex Senior Cup after conceding a 90th-minute goal against Lewes.
Kane Diedrick-Roberts struck for the visitors in the last minute on Wednesday to seal a 3-2 victory after Chi had briefly led 2-1 with 15 minutes to go.
Lewes went ahead just before half-time when James Hammond converted a low cross from Peter Gregory.
Emmett Dunn levelled for Chi in the 64th minute when he finished coolly after being put through.
Josh Clack put Chi ahead after 75 minutes when he fired in after beating his man.
The lead was short-lived as a minute later the Rooks responded with Hammond again finishing from inside the six-yard box from a Gregory cross.
An Alex Malins pass set up Diedrick-Roberts for the winner against a tired Chi.
Boss Miles Rutherford had freshened up his side after the physical and mental exertions of Tranmere but still fielded seven players who gave their all at Prenton Park in the starting XI.
Chichester go to the Pilot Field tomorrow to face Chris Agutter’s BetVictor South East leaders Hastings United.
Boss Rutherford was confident there would be no FA Cup hangover in terms of application after the Tranmere game and Wednesday’s display backed that up.
Rutherford said: “We have great spirit in this group, and that doesn’t worry me too much, and I think we shall be able to cope.”
“We have another big game on Saturday against Hastings.
“It’s not the ideal time to be playing them and Lewes but we’re not going to worry too much, we’ve just got to get on with it.”